Optimization of Ph and Temperature for Deoxyribonuclease Producing Bacteria Obtained from Soil
نویسنده
چکیده
The source of DNA for Deoxyribonuclease (DNase) producing bacteria in soil is the decaying plant cells, dead animal and bial cells. Very few organisms produce DNase extracellularly. Soil samples from different areas of Akola district (India) were screened for deoxyribonuclease production. Of the 100 soil samples screened; 10 efficient deoxyribonuclease producing bacteria were selected for morphological and biochemical characterization. These cultures were grown in nutrient broth containing DNA at various pH and temperature. The pH chosen were 3,5,7,9 and 10 and temperatures chosen were 10°C, 20°C, 30°C, 40°C and 50°C. The broth was centrifuged to separate out the cells from the above mentioned tubes. The supernatant was taken as a source of deoxyribonuclease enzyme. Each pH and temperature tube was then assayed for DNA degradation. Tubes showing maximum amount of DNA degradation in the respective pH and temperature tube was chosen as the optimum pH and temperature. In order to maximize the enzyme concentration it is necessary to optimize the conditions around cell.
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